SRA STUDY
|
SRA Study Id
|
ERP022616
(Link to NCBI )
|
Study Title
|
A benzene-degrading nitrate-reducing microbial consortium displays aerobic and anaerobic benzene degradation pathways
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SRA Experiments
|
SRA Experiment Id |
ERX1998641
(Link to NCBI ) |
Experiment Title |
Illumina HiSeq 2500 sequencing |
Exp Library Strategy |
RNA_SEQ |
Library Source |
METATRANSCRIPTOMIC |
Library Selection |
unspecified |
Library Name |
unspecified |
Library Layout |
SINGLE |
Library Instrument |
ILLUMINA_HI_SEQ_2500 |
|
|
SRA Experiment Id |
ERX1998640
(Link to NCBI ) |
Experiment Title |
Illumina HiSeq 2500 sequencing |
Exp Library Strategy |
RNA_SEQ |
Library Source |
METATRANSCRIPTOMIC |
Library Selection |
unspecified |
Library Name |
unspecified |
Library Layout |
SINGLE |
Library Instrument |
ILLUMINA_HI_SEQ_2500 |
|
|
SRA Experiment Id |
ERX1998636
(Link to NCBI ) |
Experiment Title |
Illumina HiSeq 2500 sequencing |
Exp Library Strategy |
RNA_SEQ |
Library Source |
METATRANSCRIPTOMIC |
Library Selection |
unspecified |
Library Name |
unspecified |
Library Layout |
SINGLE |
Library Instrument |
ILLUMINA_HI_SEQ_2500 |
|
|
SRA Experiment Id |
ERX1998638
(Link to NCBI ) |
Experiment Title |
Illumina HiSeq 2500 sequencing |
Exp Library Strategy |
RNA_SEQ |
Library Source |
METATRANSCRIPTOMIC |
Library Selection |
unspecified |
Library Name |
unspecified |
Library Layout |
SINGLE |
Library Instrument |
ILLUMINA_HI_SEQ_2500 |
|
|
SRA Experiment Id |
ERX1998637
(Link to NCBI ) |
Experiment Title |
Illumina HiSeq 2500 sequencing |
Exp Library Strategy |
RNA_SEQ |
Library Source |
METATRANSCRIPTOMIC |
Library Selection |
unspecified |
Library Name |
unspecified |
Library Layout |
SINGLE |
Library Instrument |
ILLUMINA_HI_SEQ_2500 |
|
|
SRA Experiment Id |
ERX1998639
(Link to NCBI ) |
Experiment Title |
Illumina HiSeq 2500 sequencing |
Exp Library Strategy |
RNA_SEQ |
Library Source |
METATRANSCRIPTOMIC |
Library Selection |
unspecified |
Library Name |
unspecified |
Library Layout |
SINGLE |
Library Instrument |
ILLUMINA_HI_SEQ_2500 |
|
Study Abstract
|
In this study, we report transcription of genes involved in aerobic and anaerobic benzene degradation pathways in a benzene-degrading denitrifying continuous culture. Transcripts associated with the family Peptococcaceae dominated all samples (21-36% relative abundance) indicating their key role in the community. We found a highly transcribed gene cluster encoding a presumed anaerobic benzene carboxylase (AbcA and AbcD) and a benzoate-coenzyme A ligase (BzlA). Predicted gene products showed >96% amino acid identity and similar gene order to the corresponding benzene degradation gene cluster described previously, providing further evidence for anaerobic benzene activation via carboxylation. For subsequent benzoyl-CoA dearomatization, bam-like genes analogous to the ones found in other strict anaerobes were transcribed, whereas gene transcripts involved in downstream benzoyl-CoA degradation were mostly analogous to the ones described in facultative anaerobes. The concurrent transcription of genes encoding enzymes involved in oxygenase-mediated aerobic benzene degradation suggested oxygen presence in the culture, possibly formed via a recently identified nitric oxide dismutase (Nod). Although we were unable to detect transcription of Nod-encoding genes, addition of nitrite and formate to the continuous culture showed indication for oxygen production. Such an oxygen production would enable aerobic microbes to thrive in oxygen-depleted and nitrate-containing subsurface environments contaminated with hydrocarbons.
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Alias
|
ena-STUDY-WAGENINGEN UNIVERSIT-14-04-2017-17:00:06:484-467
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External Id
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BioProject=PRJEB20461
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