SRA STUDY
SRA Study Id ERP009551
Study Title A genomic sequence and expression diversity catalogue of BCG
SRA Experiments
SRA Experiment Id ERX709490
Experiment Title Illumina HiSeq 2000 paired end sequencing
Exp Library Strategy WGS
Library Source GENOMIC
Library Selection RANDOM
Library Name unspecified
Library Layout PAIRED
Library Instrument ILLUMINA_HI_SEQ_2000
   
SRA Experiment Id ERX709491
Experiment Title Illumina HiSeq 2000 paired end sequencing
Exp Library Strategy WGS
Library Source GENOMIC
Library Selection RANDOM
Library Name unspecified
Library Layout PAIRED
Library Instrument ILLUMINA_HI_SEQ_2000
   
SRA Experiment Id ERX709492
Experiment Title Illumina HiSeq 2000 paired end sequencing
Exp Library Strategy WGS
Library Source GENOMIC
Library Selection RANDOM
Library Name unspecified
Library Layout PAIRED
Library Instrument ILLUMINA_HI_SEQ_2000
   
SRA Experiment Id ERX709493
Experiment Title Illumina HiSeq 2000 paired end sequencing
Exp Library Strategy WGS
Library Source GENOMIC
Library Selection RANDOM
Library Name unspecified
Library Layout PAIRED
Library Instrument ILLUMINA_HI_SEQ_2000
   
SRA Experiment Id ERX709494
Experiment Title Illumina HiSeq 2000 paired end sequencing
Exp Library Strategy WGS
Library Source GENOMIC
Library Selection RANDOM
Library Name unspecified
Library Layout PAIRED
Library Instrument ILLUMINA_HI_SEQ_2000
   
SRA Experiment Id ERX709495
Experiment Title Illumina HiSeq 2000 paired end sequencing
Exp Library Strategy WGS
Library Source GENOMIC
Library Selection RANDOM
Library Name unspecified
Library Layout PAIRED
Library Instrument ILLUMINA_HI_SEQ_2000
   
SRA Experiment Id ERX709496
Experiment Title Illumina HiSeq 2000 paired end sequencing
Exp Library Strategy WGS
Library Source GENOMIC
Library Selection RANDOM
Library Name unspecified
Library Layout PAIRED
Library Instrument ILLUMINA_HI_SEQ_2000
   
SRA Experiment Id ERX709486
Experiment Title Illumina HiSeq 2000 paired end sequencing
Exp Library Strategy WGS
Library Source GENOMIC
Library Selection RANDOM
Library Name unspecified
Library Layout PAIRED
Library Instrument ILLUMINA_HI_SEQ_2000
   
SRA Experiment Id ERX709497
Experiment Title Illumina HiSeq 2000 paired end sequencing
Exp Library Strategy WGS
Library Source GENOMIC
Library Selection RANDOM
Library Name unspecified
Library Layout PAIRED
Library Instrument ILLUMINA_HI_SEQ_2000
   
SRA Experiment Id ERX709487
Experiment Title Illumina HiSeq 2000 paired end sequencing
Exp Library Strategy WGS
Library Source GENOMIC
Library Selection RANDOM
Library Name unspecified
Library Layout PAIRED
Library Instrument ILLUMINA_HI_SEQ_2000
   
SRA Experiment Id ERX709498
Experiment Title Illumina HiSeq 2000 paired end sequencing
Exp Library Strategy WGS
Library Source GENOMIC
Library Selection RANDOM
Library Name unspecified
Library Layout PAIRED
Library Instrument ILLUMINA_HI_SEQ_2000
   
SRA Experiment Id ERX709499
Experiment Title Illumina HiSeq 2000 paired end sequencing
Exp Library Strategy WGS
Library Source GENOMIC
Library Selection RANDOM
Library Name unspecified
Library Layout PAIRED
Library Instrument ILLUMINA_HI_SEQ_2000
   
SRA Experiment Id ERX709488
Experiment Title Illumina HiSeq 2000 paired end sequencing
Exp Library Strategy WGS
Library Source GENOMIC
Library Selection RANDOM
Library Name unspecified
Library Layout PAIRED
Library Instrument ILLUMINA_HI_SEQ_2000
   
SRA Experiment Id ERX709489
Experiment Title Illumina HiSeq 2000 paired end sequencing
Exp Library Strategy WGS
Library Source GENOMIC
Library Selection RANDOM
Library Name unspecified
Library Layout PAIRED
Library Instrument ILLUMINA_HI_SEQ_2000
Study Abstract Although the Bacillus Calmette-Guérin (BCG) vaccines against tuberculosis have been available for more than 90 years, their effectiveness has been hindered by variable protective efficacy and a lack of lasting memory responses. One factor in this variability may be the diversity of the BCG strains that are used around the world, particularly the changes in the genomic material and the resulting differences in gene expression. We have compared the genomes and transcriptomes of a global collection of fourteen of the most widely used BCG strains at single base-pair resolution. We have also used quantitative proteomics to identify key differences in expression at the protein level across five representative BCG strains of the four tandem duplication groups. We provide a comprehensive map of single nucleotide polymorphisms (SNPs), copy number variation and indels across fourteen BCG strains. The distribution of SNPs across the BCG lineages allowed clustering of these strains to generate a linear phylogeny and refining the previous genealogies of BCG strains. Transcriptional and proteomic profiling revealed a metabolic remodeling in BCG strains that may reflect in altered immunogenicity and possibly vaccine efficacy. Together, the presented datasets represent the most comprehensive catalogue of genomic variation across a global collection of BCG strains.
Alias ena-STUDY-KAUST-17-02-2015-12:10:08:302-4
External Id PRJEB8560