SRA EXPERIMENT
SRA Experiment Id ERX4112978  (Link to NCBI )
Experiment Title unspecified paired end sequencing; Raw reads: ERS4558934_ilmn2
SRA Study
SRA Study Id ERP118964  (Link to NCBI )
Study Title Combined prebiotic and microbial intervention improves oral cholera vaccination responses in a mouse model of childhood undernutrition
Study Abstract Undernourished children in low-income countries often exhibit poor responses to oral vaccination. Perturbed microbiota development is linked to undernutrition but whether and how microbiota changes affect vaccine responsiveness remain unclear. Here, we show that gnotobiotic mice colonized with microbiota from undernourished Bangladeshi children and fed a Bangladeshi diet exhibited microbiota- dependent differences in mucosal IgA responses to oral vaccination with cholera toxin (CT). Supplementation with a nutrient-rich diet consisting of spirulina, amaranth, flaxseed and micronutrients augmented CT-IgA production. Mice initially colonized with a microbiota associated with poor CT responses exhibited improved immunogenicity upon invasion of bacterial taxa from cagemates colonized with a more 'responsive' microbiota. Additionally, a consortium of five cultured bacterial invaders conferred augmented CT-IgA responses in mice fed the supplemented diet and colonized with the 'hypo- responsive' community. These results provide preclinical proof-of-concept that diet and microbiota influence mucosal immune responses to CT vaccination and identify a candidate synbiotic formulation.
Alias ena-STUDY-Center for Gut Microbiome and Nutrition Research, Washington University School of Medicine-16-12-2019-16:30:05:069-1359
External Id PRJEB35845
SRA Sample
SRA Sample Id ERS4558934  (Link to NCBI )
Title Human fecal bacterial isolate annotated genome sequence
SRA Run
SRA Run Id ERR4147228  (Link to NCBI )
Spots 10804681
Bases 3241404300
Size 1534241508
Published Date 2020-05-15
Exp Library Strategy WGS
Library Source GENOMIC
Library Selection RANDOM
Library Name
Library Layout PAIRED
Library Instrument
Exp. Description
Spot Length