SRA EXPERIMENT
SRA Experiment Id ERX3515041  (Link to NCBI )
Experiment Title MinION sequencing
SRA Study
SRA Study Id ERP116996  (Link to NCBI )
Study Title Metagenomic analysis of planktonic riverine microbial consortia using nanopore sequencing reveals urban influence on river ecology
Study Abstract Riverine ecosystems are biogeochemical powerhouses driven largely by microbial communities that inhabit water columns and sediments. To gain a more broad understanding of the ecological and anthropogenic implications of river microbial consortia, we investigated the metagenomes of eleven rivers across three continents using MinION™ nanopore sequencing. Up to 10 Gb of data per run were generated with average read lengths of 3.4 kb. Diversity and diagnosis of river function potential was accomplished with 0.5-1.0×106 long reads. Our observations for seven of the eleven rivers conformed to previous findings and we exposed previously unrecognized microbial biodiversity in the other four rivers. Deeper understanding that emerged is that rivers' microbial consortia and the ecological functions they fulfil did not align with geographic location but instead indicated ecological responses to urban and other anthropogenic effects, and that changes in taxa were manifest over a very short geographic space.
Alias ena-STUDY-UC SANTA CRUZ-24-08-2019-19:45:32:082-177
External Id BioProject=PRJEB34137
SRA Sample
SRA Sample Id ERS3674335  (Link to NCBI )
Title Metagenomic analysis of planktonic riverine microbial consortia using nanopore sequencing reveals urban influence on river ecology
SRA Run
SRA Run Id ERR3493785  (Link to NCBI )
Spots 4388
Bases 16261181
Size 13720903
Published Date 2019-08-29
Exp Library Strategy WGS
Library Source METAGENOMIC
Library Selection other
Library Name lab18_all_pass_barcode07_negcontrol.fastq.gz
Library Layout SINGLE
Library Instrument MIN_ION
Exp. Description
Spot Length