SRA EXPERIMENT
SRA Experiment Id ERX3190252  (Link to NCBI )
Experiment Title Illumina MiSeq sequencing; qiita_ptid_6188:11176.3x.CC0102F.G2.072516.174
SRA Study
SRA Study Id ERP113817  (Link to NCBI )
Study Title Triplicate PCR reactions for 16S rRNA gene amplicon sequencing are unnecessary
Study Abstract Conventional wisdom holds that PCR amplification for sequencing should employ pooled replicate reactions to reduce bias due to jackpot effects and chimera formation. However, modern amplicon data analysis employs methods that may be less sensitive to such artifacts. Here we directly compare results from single versus triplicate reactions for 16S amplicon sequencing and find no significant impact of adopting a less labor-intensive single-reaction protocol.
Alias qiita_sid_11176
External Id BioProject=PRJEB31281
SRA Sample
SRA Sample Id ERS3163895  (Link to NCBI )
SRA Run
SRA Run Id ERR3162168  (Link to NCBI )
Spots 461282
Bases 127383871
Size 77268334
Published Date 2020-02-07
Exp Library Strategy OTHER
Library Source METAGENOMIC
Library Selection PCR
Library Name 11176.3x.CC0102F.G2.072516.174
Library Layout SINGLE
Library Instrument ILLUMINA_MI_SEQ
Exp. Description
Spot Length