SRA EXPERIMENT
SRA Experiment Id ERX3187616  (Link to NCBI )
Experiment Title Illumina MiSeq sequencing; qiita_ptid_6189:11176.3x.1.Marine.Sediment.H9
SRA Study
SRA Study Id ERP113817  (Link to NCBI )
Study Title Triplicate PCR reactions for 16S rRNA gene amplicon sequencing are unnecessary
Study Abstract Conventional wisdom holds that PCR amplification for sequencing should employ pooled replicate reactions to reduce bias due to jackpot effects and chimera formation. However, modern amplicon data analysis employs methods that may be less sensitive to such artifacts. Here we directly compare results from single versus triplicate reactions for 16S amplicon sequencing and find no significant impact of adopting a less labor-intensive single-reaction protocol.
Alias qiita_sid_11176
External Id BioProject=PRJEB31281
SRA Sample
SRA Sample Id ERS3147426  (Link to NCBI )
SRA Run
SRA Run Id ERR3158554  (Link to NCBI )
Spots 8184
Bases 1235481
Size 850677
Published Date 2020-02-07
Exp Library Strategy OTHER
Library Source METAGENOMIC
Library Selection PCR
Library Name 11176.3x.1.Marine.Sediment.H9
Library Layout SINGLE
Library Instrument ILLUMINA_MI_SEQ
Exp. Description
Spot Length