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SRA EXPERIMENT
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SRA Experiment Id
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ERX3187522
(Link to NCBI )
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Experiment Title
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Illumina MiSeq sequencing; qiita_ptid_6189:11176.1x.4.Marine.Water.B10
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SRA Study
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| SRA Study Id |
ERP113817
(Link to NCBI ) |
| Study Title |
Triplicate PCR reactions for 16S rRNA gene amplicon sequencing are unnecessary |
| Study Abstract |
Conventional wisdom holds that PCR amplification for sequencing should employ pooled replicate reactions to reduce bias due to jackpot effects and chimera formation. However, modern amplicon data analysis employs methods that may be less sensitive to such artifacts. Here we directly compare results from single versus triplicate reactions for 16S amplicon sequencing and find no significant impact of adopting a less labor-intensive single-reaction protocol. |
| Alias |
qiita_sid_11176 |
| External Id |
BioProject=PRJEB31281 |
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SRA Sample
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SRA Run
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Exp Library Strategy
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OTHER
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Library Source
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METAGENOMIC
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Library Selection
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PCR
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Library Name
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11176.1x.4.Marine.Water.B10
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Library Layout
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SINGLE
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Library Instrument
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ILLUMINA_MI_SEQ
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Exp. Description
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Spot Length
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