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Study

Study Name: Environmental and plant-associated microbial communities from various locations in the United States

Projects

Biosamples

Organisms

Biosamples: 504 Seq. Projects: 504
STUDY INFORMATION
GOLD Study ID Gs0154289
Study Name Environmental and plant-associated microbial communities from various locations in the United States
Other Names
NCBI Umbrella Bioproject Name
NCBI Umbrella Bioproject ID
SRA Studies
SRA Study Id ERP113817  (Link to NCBI )
Study Title Triplicate PCR reactions for 16S rRNA gene amplicon sequencing are unnecessary
Study Abstract Conventional wisdom holds that PCR amplification for sequencing should employ pooled replicate reactions to reduce bias due to jackpot effects and chimera formation. However, modern amplicon data analysis employs methods that may be less sensitive to such artifacts. Here we directly compare results from single versus triplicate reactions for 16S amplicon sequencing and find no significant impact of adopting a less labor-intensive single-reaction protocol.
  
Legacy ER Study ID
Legacy GOLD ID
Added By JGI automated process on 2021-07-27
Last Modified By
PI
Description Environmental and plant-associated microbial communities from various locations in the United States
Relevance
Study Information Visibility Public
Metagenomic Study Yes
Publication
Is GEBA
Is HMP
ECOSYSTEM CLASSIFICATION
Ecosystem Host-associated
Ecosystem Category Plants
Ecosystem Type Roots
Ecosystem Subtype Unclassified
Specific Ecosystem Unclassified
STUDY COMPOSITION
Number of Biosamples 504
Number of Organisms 0
Number of Seq Projects 504
Number of Analysis Projects 0
Number of Related Studies 0

 

 

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