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Study

Study Name: Cultivation and metabarcoding of bacteria associated with Ectocarpus subulatus cultures

Projects

Biosamples

Organisms

Biosamples: 0 Seq. Projects: 10
STUDY INFORMATION
GOLD Study ID Gs0148965
Study Name Cultivation and metabarcoding of bacteria associated with Ectocarpus subulatus cultures
Other Names
NCBI Umbrella Bioproject Name
NCBI Umbrella Bioproject Accession
SRA Studies
Legacy ER Study ID
Legacy GOLD ID
Added By JGI automated process on 2020-07-06
Last Modified By
PI
Description This project aims to elucidate the culturable and unculturable bacterial microbiome of Ectocarpus subulatus (Phaeophyceae) cultures. Bacteria were isolated by direct plating and by dilution to extinction techniques from ground algal material and culture medium after a range of pretreatments (grinding, cell wall digestion, filtration, antibiotic treatments), and in different media. Each treatment was combined with different solid/liquid culture media (Zobell, R2A, LNHM, etc.), and obtained sequences barcoded by Sanger-sequencing of the 16S. Metabarcoding of the bacterial community was carried out by Illumina 16S-Miseq sequencing of selected cultures. Selected bacteria were genome-sequenced using paired-end Illumina Miseq sequencing.
Relevance
Study Information Visibility Public
Metagenomic Study No
Publication
Is GEBA
Is HMP
ECOSYSTEM CLASSIFICATION
Ecosystem
Ecosystem Category
Ecosystem Type
Ecosystem Subtype
Specific Ecosystem
STUDY COMPOSITION
Number of Biosamples 0
Number of Organisms 10
Number of Seq Projects 10
Number of Analysis Projects 10
Number of Related Studies 0

 

 

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