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Study

Study Name: Anaerobic granular sludge microbial community from Brazil

Projects

Biosamples

Organisms

Biosamples: 6 Seq. Projects: 6
STUDY INFORMATION
GOLD Study ID Gs0145101
Study Name Anaerobic granular sludge microbial community from Brazil
Other Names
NCBI Umbrella Bioproject Name
NCBI Umbrella Bioproject ID
SRA Studies
SRA Study Id ERP107967  (Link to NCBI )
Study Title Comparative metatranscriptomic analysis of anaerobic digesters treating anionic surfactant contaminated wastewater
Study Abstract A metatranscriptomic approach was applied to reveal active biological functions and their assigned taxonomic profiles in complex microbial communities of anaerobic reactors applied to anionic surfactant degradation. For this purpose, three laboratory scale upflow anaerobic sludge blanket (UASB) reactors under different feeding types were compared. The feedings were synthetic medium (UASB_Control), synthetic medium and linear alkylbenzene sulfonate (LAS; UASB_SL), and real laundry wastewater (UASB_LW). A homemade bioinformatics pipeline combined to an R workflow was developed to perform trimming, co-assembling, gene calling, mapping, assignment and statistical analyses of metatranscriptomic datasets. UASB_SL and UASB_LW showed similar percentages of LAS removal (~50 %). UASB_SL showed the greatest richness and metabolic activity, followed by UASB_LW and UASB_Control. Rarefaction analysis using KO numbers revealed that 1-2 million reads were sufficient to access the functional capacity of the studied microbiomes. Genes and taxonomies associated in all steps of anaerobic LAS biodegradation were observed with high relative abundance in the UASB_SL and UASB_LW than UASB_Control. Therefore, our data corroborates the fumarate addition as the activation mechanism to initiate the removal of LAS by fumarate reductase enzyme assigned to the Syntrophobacter genus. The beta-oxidation step could be performed by Smithella, Acinetobacter and Syntrophorhabdus, whereas, for the ring cleavage a 6-OCH-CoA hydrolase attributed to the genus Desulfomonile and Syntrophorhabdus were found. In the last step, desulfonation, were observed the active genera Desulfovibrio and Desulfomonile. Thus, the integration of physicochemical results from the biological reactors and the taxonomy and functional annotations of mRNA provides a powerful tool to understand the microbial consortium involved in the biodegradation of LAS.
  
Legacy ER Study ID
Legacy GOLD ID
Added By JGI automated process on 2020-01-02
Last Modified By
PI
Description Anaerobic granular sludge microbial community from Brazil
Relevance
Study Information Link
Study Information Visibility Public
Metagenomic Study Yes
Publication
Is GEBA
Is HMP
ECOSYSTEM CLASSIFICATION
Ecosystem Engineered
Ecosystem Category Wastewater
Ecosystem Type Nutrient removal
Ecosystem Subtype Dissolved organics (anaerobic)
Specific Ecosystem Unclassified
STUDY COMPOSITION
Number of Biosamples 6
Number of Organisms 0
Number of Seq Projects 6
Number of Analysis Projects 2
Number of Related Studies 0

 

 

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