Studies
|
56,036 |
|
Biosamples
|
191,586 |
|
Sequencing Projects
|
515,942 |
|
Analysis Projects
|
381,633 |
| Organisms | 480,439 |
| PROJECT INFORMATION | |
|---|---|
|
GOLD Project ID
|
Gp0144661
|
|
Project Name
|
Escherichia coli C41(DE3)
|
|
Other Names
|
|
|
Legacy GOLD ID
|
|
| NCBI BioProject Name | Escherichia coli strain:C41(DE3) |
|
NCBI BioProject Accession
|
PRJNA273434 |
|
NCBI Locus Tag
|
TH69
|
|
NCBI BioSample Accession
|
SAMN03292179 |
|
Source Sample ID
|
|
| PI | Unknown |
| Added By | JGI automated process on 2016-05-18 |
| Last Modified By | Supratim Mukherjee on 2016-05-18 |
| Project Comments |
|
|
Project Status
|
Complete and Published
|
|
Project Relevance
|
Industrial |
|
Sequencing Center
|
Stockholm University
(Sweden)
|
|
Collaborating Institute
|
|
|
Funding Agency
|
|
|
Project Description
|
The Escherichia coli strain C41(DE3) was derived from BL21(DE3) for its improved ability to produce recombinant proteins (see: J Mol Biol. 1996 Jul 19;260(3):289-98). This study aims at identifying and understanding the genetic adaptations that have occurred during the isolation of C41(DE3). Such knowledge may provide leads for future studies aimed at the isolation of improved protein production mutants, and help to facilitate recombinant protein production in Escherichia coli.
|
| Is JGI Project |
No
|
|
Project Information Visibility
|
Public |
| PROJECT TYPE | |
|
Specimen
|
Organism |
|
Nucleic Acid
|
DNA |
|
Sequencing Strategy
|
Whole Genome Sequencing
|
| EXTERNAL PROJECT LINKS | |
|
JGI Data Utilization Status
|
|
|
JGI Award DOI
|
|
|
Genome Publications
|
|
|
Other Publications
|
|
|
Ignored Publications
|
|
|
Unassigned Publications
|
|
| SRA Experiment |
|
| PROJECT COMPOSITION | |
| Study |
Escherichia coli C41(DE3) Genome sequencing - Stockholm University |
| Organism |
Escherichia coli C41(DE3) |
| Analysis Projects |
Escherichia coli C41(DE3) |
| Number of Analysis Projects (AP) |
1 |
| Number of Derived Analyis Projects (DAP) |
0
|
| SEQUENCING INFORMATION | |
|---|---|
|
Sequencing Status
|
Complete |
| Sequencing Quality (MIGS-31) |
Level 6: Finished |
|
Finishing Goal
|
|
|
Contamination Screening Input
|
|
|
DNA Amplification Method
|
|
|
Single Cell Lysis Protocol
|
|
|
Single Cell Lysis Approach
|
|
|
Sequencing Comments
|
|
|
DNA Amplification Kit
|
|
|
Sequencing Technology
(MIGS-29)
|
Sanger 454 |
|
Library Method
(MIGS-28)
|
|
|
Library Layout
|
|
|
Library Screening Method
|
|
|
Number Of Reads
|
|
|
Read Size
|
|
|
Vector
|
|
| GC Percent |
|
| Chromosome Count |
|
| Plasmid Count |
|
| ORGANISM NAME | |
|---|---|
| GOLD Organism ID |
Go0117599 |
| Organism Name |
Escherichia coli C41(DE3)
|
| Other names (alias, synonyms, short names, common names) |
|
| Organism Domain |
BACTERIAL
|
| Phylogeny |
PROTEOBACTERIA-GAMMA
|
| Genus |
Escherichia
|
| Genus Synonyms |
|
| Species |
Escherichia coli
|
| Subspecies |
|
| Species Synonyms |
|
| Strain |
C41(DE3)
|
| Strain Synonyms |
|
| Serovar/Cultivar |
|
| Culture Collection ID |
|
| Type Strain |
|
| Exemplar DOI |
|
| Exemplar Name |
|
| Taxon DOI |
|
| Biosafety Level |
|
| Organism Comments |
|
| Cultured |
Yes
|
| Culture Type |
Isolate
|
| Organism Type |
Natural
|
| Uncultured Type |
|
| Commercial Strain |
|
| Commercial Strain Comments |
|
|
ORGANISM TAXONOMY
|
|
| NCBI Taxonomy ID |
562 |
| NCBI Superkingdom |
Bacteria
|
| NCBI Kingdom |
|
| NCBI Phylum |
Pseudomonadota
|
| NCBI Class |
Gammaproteobacteria
|
| NCBI Order |
Enterobacterales
|
| NCBI Family |
Enterobacteriaceae
|
| NCBI Genus |
Escherichia
|
| NCBI Species |
Escherichia coli
|
| ISOLATION CLASSIFICATION | |
| Ecosystem |
|
| Ecosystem Category |
|
| Ecosystem Type |
|
| Ecosystem Subtype |
|
| Specific Ecosystem |
|
| ORGANISM EXTERNAL REFERENCES | |
| Strain Info ID |
|
| Genbank 16S ID |
|
| GENERAL PROPERTIES | |
|---|---|
| Oxygen Requirement |
|
| Cell Shape |
|
| Motility |
|
| Sporulation |
|
| Temperature Range |
|
| Salinity |
|
| pH |
|
| Cell Diameter |
|
| Cell Length |
|
| Color |
|
| Gram Staining |
|
| Biotic Relationships |
|
| Symbiotic Physical Interaction |
|
| Symbiotic Relationship |
|
| Symbiont Name |
|
| Symbiont Taxon ID |
|
| Cell Arrangements |
|
| Diseases |
|
| Known Habitats |
|
| Metabolisms |
|
| Phenotypes |
|
| Energy Sources |
|
You are going to add the following user to your project, biosample and study.
Contact Name/Email: Do you want to continue?
You are going to remove permissions for the following user to your project, biosample and study.
Contact Name/Email: Do you want to continue?
Changing the SP visibility will also change visibility on the following items:
Do you want to continue with this visibility change?
Checking that this Sequencing Project can be made private:
Do you want to continue with this visibility change?
Changing the SP Sequencing Strategy will also effect these items:
Do you want to continue with this Sequencing Strategy change?
