PROJECT INFORMATION
GOLD Project ID
Gp0093792  
Project Name
Thermoanaerobaculum aquaticum MP-01  
Other Names
  
Legacy ER Project ID
73635  
Legacy GOLD ID
Gi0073635  
NCBI BioProject Name Thermoanaerobaculum aquaticum strain:MP-01
NCBI BioProject ID 245240
NCBI BioProject Accession PRJNA245240
NCBI Locus Tag
EG19  
NCBI BioSample Accession SAMN02732492
PI
Added By JGI automated process on 2014-05-05
Last Modified By JGI automated process on 2014-08-18
Project Comments
  
Project Status
Permanent Draft  
Project Relevance Environmental
Sequencing Center Oklahoma Medical Research Foundation
University of Oklahoma (USA)
Collaborating Institute
  
Funding Agency
  
Project Description
A novel bacterium was isolated from a freshwater hot spring, the Hale House Spring, located at Hot Springs National Park, Hot Springs, AR, USA. Cells of strain MP-01T stained Gram-negative, were rod-shaped, non-motile, strictly anaerobic and chemo-organotrophic and did not form spores. Growth occurred at 50–65 °C, with an optimum at 60 °C, at pH 6.0–8.0, with an optimum at pH 6.5–7.0, and at NaCl concentrations up to 0.5 % (w/v), with optimum growth in the absence of NaCl. Strain MP-01T was capable of fermentative growth on pyruvate or proteinaceous substrates as well as reducing Fe(III) and Mn(IV). Major fatty acids were iso-C15 : 0, iso-C16 : 0, anteiso-C17 : 0 and iso-C17 : 0. The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine and the major isoprenoid quinone was MK-10. In the polyamine pattern, sym-homospermidine was the predominant compound. The DNA G+C content was 62.7 mol%. Analysis of the 16S rRNA gene sequence of the isolate indicated that strain MP-01T represents the first reported cultivated member of subdivision 23 of the Acidobacteria. It is proposed that strain MP-01T represents a novel genus and species, for which the name Thermoanaerobaculum aquaticum gen. nov., sp. nov. is proposed. The type strain of Thermoanaerobaculum aquaticum is MP-01T ( = DSM 24856T = JCM 18256T).  
Is JGI Project
No  
Project Information Visibility Public
PROJECT TYPE
Specimen Organism
Nucleic Acid DNA
Sequencing Strategy
Whole Genome Sequencing  
EXTERNAL PROJECT LINKS
Project Information
  
Database
  
Genome Publications
  
Other Publications
  
SRA Experiment
 
SRA Experiment Id SRX525356  (Link to NCBI )
Experiment Title Thermoanaerobaculum aquaticum Genome sequencing
Exp Library Strategy WGS
Library Source GENOMIC
Library Selection unspecified
Library Name MP01
Library Layout PAIRED
Library Instrument ILLUMINA_MI_SEQ
Spot Length 300
PROJECT COMPOSITION
Study Thermoanaerobaculum aquaticum strain:MP-01 Genome sequencing
Organism Thermoanaerobaculum aquaticum MP-01
Analysis Projects Thermoanaerobaculum aquaticum MP-01
Number of Analysis Projects (AP)
1  
Number of Derived Analyis Projects (DAP)
0  
SEQUENCING INFORMATION
Sequencing Status Complete
Sequencing Quality (MIGS-31)
Finishing Goal
  
Sequencing Comments
  
Sequencing Technology (MIGS-29) Illumina MiSeq
Library Method (MIGS-28)
  
Number Of Reads
  
Read Size
  
Vector
  
GC Percent
63  
Chromosome Count
  
Plasmid Count
  
ORGANISM NAME
GOLD Organism ID Go0093792
Organism Name
Thermoanaerobaculum aquaticum MP-01  
Other names (alias, synonyms, short names, common names)
  
Organism Domain
BACTERIAL  
Phylogeny
ACIDOBACTERIA  
Genus
Thermoanaerobaculum  
Genus Synonyms
  
Species
Thermoanaerobaculum aquaticum  
Subspecies
  
Species Synonyms
  
Strain
MP-01  
Strain Synonyms
  
Serovar/Cultivar
  
Culture Collection ID
  
Type Strain
Yes  
Exemplar DOI
10.1601/ex.24795  
Exemplar Name
Thermoanaerobaculum aquaticum  
Taxon DOI
10.1601/tx.24795  
Biosafety Level
  
Organism Comments
  
Cultured
Yes  
Culture Type
Isolate  
Organism Type
Natural  
Uncultured Type
  
Commercial Strain
  
Commercial Strain Comments
  
ORGANISM TAXONOMY
NCBI Taxonomy ID 1312852
NCBI Superkingdom
Bacteria  
NCBI Kingdom
  
NCBI Phylum
Acidobacteria  
NCBI Class
Thermoanaerobaculia  
NCBI Order
Thermoanaerobaculales  
NCBI Family
Thermoanaerobaculaceae  
NCBI Genus
Thermoanaerobaculum  
NCBI Species
Thermoanaerobaculum aquaticum  
ISOLATION CLASSIFICATION
Ecosystem
  
Ecosystem Category
  
Ecosystem Type
  
Ecosystem Subtype
  
Specific Ecosystem
  
ORGANISM EXTERNAL REFERENCES
Strain Info ID
Genbank 16S ID
GENERAL PROPERTIES
Oxygen Requirement
  
Cell Shape
  
Motility
  
Sporulation
  
Temperature Range
  
Salinity
  
pH
  
Cell Diameter
  
Cell Length
  
Color
  
Gram Staining
  
Biotic Relationships
  
Symbiotic Physical Interaction
  
Symbiotic Relationship
  
Symbiont Name
  
Symbiont Taxon ID
  
Cell Arrangements
  
Diseases
  
Known Habitats
  
Metabolisms
  
Phenotypes
  
Energy Sources
  

 

 

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