PROJECT INFORMATION
GOLD Project ID
Gp0083214  
Project Name
Tetrasphaera jenkinsii Ben 74  
Other Names
  
Legacy ER Project ID
67778  
Legacy GOLD ID
Gi0067778  
NCBI BioProject Name Tetrasphaera jenkinsii Ben 74
NCBI BioProject ID 215314
NCBI BioProject Accession PRJEB37
NCBI Locus Tag
BN13  
NCBI BioSample Accession SAMEA3146295
PI
Added By JGI automated process on 2014-01-23
Last Modified By Supratim Mukherjee on 2017-03-29
Project Comments
  
Project Status
Permanent Draft  
Project Relevance
  
Sequencing Center Aalborg University (Denmark)
Collaborating Institute
  
Funding Agency
  
Project Description
Members of the genus Tetrasphaera are considered to be putative polyphosphate accumulating organisms (PAOs) in enhanced biological phosphorus removal (EBPR) from wastewater. Although abundant in Danish full-scale wastewater EBPR plants, how similar their ecophysiology is to Candidatus 'Accumulibacter phosphatis' is unclear, although they may occupy different ecological niches in EBPR communities. The genomes of four Tetrasphaera isolates (T. australiensis, T. japonica, T. elongata, and T. jenkinsii) were sequenced and annotated, and the data used to construct metabolic models. These models incorporate central aspects of carbon and phosphorus metabolism critical to understanding their behaviour under the alternating anaerobic/aerobic conditions encountered in EBPR plants. Key features of these metabolic pathways were investigated in pure cultures, although poor growth limited their analyses to T. japonica and T. elongata. Based on the models, we propose that under anaerobic conditions the Tetrasphaera-related PAOs take up glucose and ferment this to succinate and other components. They also synthesise glycogen as a storage polymer, using energy generated from the degradation of stored polyphosphate and substrate fermentation. During the aerobic phase, the stored glycogen is catabolized to provide energy for growth and to replenish the intracellular polyphosphate reserves needed for subsequent anaerobic metabolism. They are also able to denitrify. This physiology is markedly different to that displayed by 'Candidatus Accumulibacter phosphatis', and reveals Tetrasphaera populations to be unusual and physiologically versatile PAOs carrying out denitrification, fermentation and polyphosphate accumulation.  
Is JGI Project
No  
Project Information Visibility Public
PROJECT TYPE
Specimen Organism
Nucleic Acid DNA
Sequencing Strategy
Whole Genome Sequencing  
EXTERNAL PROJECT LINKS
Project Information
  
Database
  
Genome Publications
  
Other Publications
  
SRA Experiment
  
PROJECT COMPOSITION
Study A metabolic model for Tetrasphaera jenkinsii in wastewater treatment
Organism Tetrasphaera jenkinsii Ben 74
Analysis Projects Tetrasphaera jenkinsii Ben 74
Number of Analysis Projects (AP)
1  
Number of Derived Analyis Projects (DAP)
0  
SEQUENCING INFORMATION
Sequencing Status Complete
Sequencing Quality (MIGS-31)
Finishing Goal
  
Sequencing Comments
  
Sequencing Technology (MIGS-29)
  
Library Method (MIGS-28)
  
Number Of Reads
  
Read Size
  
Vector
  
GC Percent
  
Chromosome Count
  
Plasmid Count
  
ORGANISM NAME
GOLD Organism ID Go0083214
Organism Name
Tetrasphaera jenkinsii Ben 74  
Other names (alias, synonyms, short names, common names)
  
Organism Domain
BACTERIAL  
Phylogeny
ACTINOBACTERIA  
Genus
Tetrasphaera  
Genus Synonyms
  
Species
Tetrasphaera jenkinsii  
Subspecies
  
Species Synonyms
  
Strain
Ben 74  
Strain Synonyms
  
Serovar/Cultivar
  
Culture Collection ID
DSM 17519  
Type Strain
Yes  
Exemplar DOI
10.1601/ex.11100  
Exemplar Name
Tetrasphaera jenkinsii  
Taxon DOI
10.1601/tx.11100  
Biosafety Level
1  
Organism Comments
  
Cultured
Yes  
Culture Type
Isolate  
Organism Type
Natural  
Uncultured Type
  
Commercial Strain
  
Commercial Strain Comments
  
ORGANISM TAXONOMY
NCBI Taxonomy ID 1193518
NCBI Superkingdom
Bacteria  
NCBI Kingdom
  
NCBI Phylum
Actinobacteria  
NCBI Class
Actinobacteria  
NCBI Order
Micrococcales  
NCBI Family
Intrasporangiaceae  
NCBI Genus
Tetrasphaera  
NCBI Species
Tetrasphaera jenkinsii  
ISOLATION CLASSIFICATION
Ecosystem
  
Ecosystem Category
  
Ecosystem Type
  
Ecosystem Subtype
  
Specific Ecosystem
  
ORGANISM EXTERNAL REFERENCES
Strain Info ID
Genbank 16S ID
GENERAL PROPERTIES
Oxygen Requirement
  
Cell Shape
  
Motility
  
Sporulation
  
Temperature Range
  
Salinity
  
pH
  
Cell Diameter
  
Cell Length
  
Color
  
Gram Staining
Gram+  
Biotic Relationships
  
Symbiotic Physical Interaction
  
Symbiotic Relationship
  
Symbiont Name
  
Symbiont Taxon ID
  
Cell Arrangements
  
Diseases
  
Known Habitats Sludge
Wastewater
Metabolisms
  
Phenotypes
  
Energy Sources
  
CYANOBACTERIA METADATA
Filaments
  
Filament Type
  
Differentiated Cells
  
Position Of Heterocysts
  
Cysts
  
Mode Of Reproduction
  
Trichome
  
Trichome Type
  
Carotenoids
  
Pigments
  
Phage Infectivity
  
Phage Type
  
Restriction Enzyme
  
Toxin Production
  
Toxin Type
  
Secondary Metabolites
  
Developmental Cycle
  
Branching
  
Gas Vesicles
  
Gas Vesicle Type
  
Diagnostic Inclusion Body Information
  
Extracellular Structures
  
Bloom Forming
  
Pigment Type
  
Complementary chromatic adaptation (CCA)
  
Medium Types
  

 

 

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