PROJECT NAME
GOLD Project ID
Gp0005034  
Project Name
Escherichia coli BL21 (DE3)  
Other Names
  
Legacy ER Project ID
14863  
Legacy GOLD ID
Gi04342, Gc01082  
NCBI BioProject Name Escherichia coli BL21-Gold(DE3)pLysS AG
NCBI BioProject ID 30681
NCBI BioProject Accession
PRJNA30681  
NCBI Locus Tag
ECBD  
NCBI BioSample Accession
SAMN00002656  
PI Eddy Rubin
Added By Nikos Kyrpides on 2009-05-12
Last Modified By Auto script update processes on 2014-05-20
Project Comments
  
PROJECT TYPE
Specimen Organism
Nucleic Acid DNA
Sequencing Strategy
Whole Genome Sequencing  
PROJECT INFORMATION
Project Status
Complete and Published  
Project Relevance Biotechnological
Recombinant protein expression
Sequencing Center DOE Joint Genome Institute (JGI) (USA)
Collaborating Institute
  
Funding Agency U.S. Department of Energy (DOE) (USA)
Project Description
Common laboratory strain used for recombinant protein expression.  
Project Information Visibility
Public  
EXTERNAL PROJECT LINKS
JGI Portal Download Raw Sequence Data
Project Information Taxonomy
Entrez
Database
  
Genome Publications
  
Other Publications
  
Isolation Publications
  
NCBI SRA ID
  
PROJECT COMPOSITION
Studies DD - Eddy Rubin
Analysis Projects Escherichia coli BL21 (DE3)
SEQUENCING INFORMATION
Sequencing Status Complete
Sequencing Quality (MIGS-31) Level 6: Finished
Finishing Goal Level 6: Finished
Sequencing Comments
  
Sequencing Technology (MIGS-29) 454-GS-FLX
Library Method (MIGS-28)
  
Number Of Reads
  
Read Size
  
Vector
  
GC Percent
51  
Chromosome Count
1  
Plasmid Count
1  
ORGANISM NAME
GOLD Organism ID Go0001402
Organism Name
Escherichia coli BL21 (DE3)  
Other names (alias, synonyms, short names, common names)
  
Organism Domain
BACTERIAL  
Phylogeny
PROTEOBACTERIA-GAMMA  
Genus
Escherichia  
Genus Synonyms
  
Species
Escherichia coli  
Subspecies
  
Species Synonyms
  
Strain
BL21 (DE3)  
Strain Synonyms
  
Serovar-Biovar
  
Culture Collection ID
ATCC BAA-1025  
Type Strain
No  
Exemplar DOI
  
Exemplar Name
  
Taxon DOI
  
Biosafety Level
1  
Organism Comments
  
Cultured
Yes  
Culture Type
Isolate  
Organism Type
Natural  
Uncultured Type
  
Commercial Strain
  
Commercial Strain Comments
  
ORGANISM TAXONOMY
NCBI Taxonomy ID 469008
NCBI Kingdom
  
NCBI Phylum
Proteobacteria  
NCBI Class
Gammaproteobacteria  
NCBI Order
Enterobacteriales  
NCBI Family
Enterobacteriaceae  
NCBI Genus
Escherichia  
NCBI Species
Escherichia coli  
ISOLATION CLASSIFICATION
Ecosystem
Engineered  
Ecosystem Category
Lab synthesis  
Ecosystem Type
Unclassified  
Ecosystem Subtype
Unclassified  
Specific Ecosystem
Unclassified  
ORGANISM EXTERNAL REFERENCES
Strain Info ID 493519
Genbank 16S ID
Synonym List
GENERAL PROPERTIES
Oxygen Requirement
Facultative  
Cell Shape
Rod-shaped  
Motility
Motile  
Sporulation
Nonsporulating  
Temperature Range
Mesophile  
Temperature Optimum
37  
Salinity
  
pH
  
Cell Diameter
  
Cell Length
  
Color
  
Gram Staining
Gram-  
Biotic Relationships
ID 3
Term Free living
Symbiotic Physical Interaction
  
Symbiotic Relationship
  
Symbiont Name
  
Symbiont Taxon ID
  
Cell Arrangements Pairs
Singles
Diseases None
Known Habitats Host
Human intestinal microflora
Metabolisms
  
Phenotypes Non-Pathogen
Energy Sources
  

 

 

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