PROJECT NAME
GOLD Project ID
Gp0001402  
Project Name
Escherichia coli BL21 (DE3)  
Other Names
  
Legacy ER Project ID
11306  
Legacy GOLD ID
Gi02360, Gc01058  
NCBI BioProject Name Escherichia coli BL21(DE3)
NCBI BioProject ID 20713
NCBI BioProject Accession PRJNA20713
NCBI Locus Tag
ECD  
NCBI BioSample Accession SAMN02603478
PI Jihyun F. Kim
Added By Nikos Kyrpides on 2007-11-27
Last Modified By Supratim Mukherjee on 2016-01-14
Project Comments
  
PROJECT TYPE
Specimen Organism
Nucleic Acid DNA
Sequencing Strategy
Whole Genome Sequencing  
PROJECT INFORMATION
Project Status
Complete and Published  
Project Relevance Biotechnological
Recombinant protein expression
Sequencing Center Korea Research Institute of Bioscience and Biotechnology (South Korea)
Collaborating Institute
  
Funding Agency
  
Project Description
Common laboratory strain used for recombinant protein expression.  
Project Information Visibility Public
EXTERNAL PROJECT LINKS
Project Information
  
Database
  
Genome Publications Journal of molecular biology 394(4):644-52 Genome sequences of Escherichia coli B strains REL606 and BL21(DE3).
Other Publications
  
PROJECT COMPOSITION
Study Escherichia coli BL21(DE3)
Analysis Projects Escherichia coli BL21 (DE3)
SEQUENCING INFORMATION
Sequencing Status Complete
Sequencing Quality (MIGS-31) Level 6: Finished
Finishing Goal Level 6: Finished
Sequencing Comments
  
Sequencing Technology (MIGS-29)
  
Library Method (MIGS-28)
  
Number Of Reads
  
Read Size
  
Vector
  
GC Percent
51  
Chromosome Count
1  
Plasmid Count
0  
ORGANISM NAME
GOLD Organism ID Go0001402
Organism Name
Escherichia coli BL21 (DE3)  
Other names (alias, synonyms, short names, common names)
  
Organism Domain
BACTERIAL  
Phylogeny
PROTEOBACTERIA-GAMMA  
Genus
Escherichia  
Genus Synonyms
  
Species
Escherichia coli  
Subspecies
  
Species Synonyms
  
Strain
BL21 (DE3)  
Strain Synonyms
  
Serovar-Biovar
  
Culture Collection ID
ATCC BAA-1025  
Type Strain
No  
Exemplar DOI
  
Exemplar Name
  
Taxon DOI
  
Biosafety Level
1  
Organism Comments
  
Cultured
Yes  
Culture Type
Isolate  
Organism Type
Natural  
Uncultured Type
  
Commercial Strain
  
Commercial Strain Comments
  
ORGANISM TAXONOMY
NCBI Taxonomy ID 469008
NCBI Superkingdom
Bacteria  
NCBI Kingdom
  
NCBI Phylum
Proteobacteria  
NCBI Class
Gammaproteobacteria  
NCBI Order
Enterobacterales  
NCBI Family
Enterobacteriaceae  
NCBI Genus
Escherichia  
NCBI Species
Escherichia coli  
ISOLATION CLASSIFICATION
Ecosystem
Engineered  
Ecosystem Category
Lab synthesis  
Ecosystem Type
Unclassified  
Ecosystem Subtype
Unclassified  
Specific Ecosystem
Unclassified  
ORGANISM EXTERNAL REFERENCES
Strain Info ID 493519
Genbank 16S ID
Synonym List
  
GENERAL PROPERTIES
Oxygen Requirement
Facultative  
Cell Shape
Rod-shaped  
Motility
Motile  
Sporulation
Nonsporulating  
Temperature Range
Mesophile  
Temperature Optimum
37  
Salinity
  
pH
  
Cell Diameter
  
Cell Length
  
Color
  
Gram Staining
Gram-  
Biotic Relationships
ID 3
Term Free living
Symbiotic Physical Interaction
  
Symbiotic Relationship
  
Symbiont Name
  
Symbiont Taxon ID
  
Cell Arrangements Singles
Pairs
Diseases None
Known Habitats Human intestinal microflora
Host
Metabolisms
  
Phenotypes Non-Pathogen
Energy Sources
  

 

 

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