PROJECT INFORMATION
GOLD Project ID
Gp0036960  
Project Name
Enterobacter sp. R4-368  
Other Names
  
Legacy ER Project ID
42832  
Legacy GOLD ID
Gi0042832, Gc0042832  
NCBI BioProject Name Enterobacter sp. R4-368
NCBI BioProject ID 189171
NCBI BioProject Accession PRJNA189171
NCBI Locus Tag
H650  
NCBI BioSample Accession
PI Madhaiyan Munusamy
Added By Tatiparthi Reddy on 2013-03-27
Last Modified By JGI automated process on 2014-08-18
Project Comments
  
Project Status
Complete and Published  
Project Relevance Agricultural
Sequencing Center Macrogen (USA)
Collaborating Institute National University of Singapore (Singapore)
Funding Agency Temasek Trust (Singapore)
Project Description
Jatropha (Jatropha curcas L.) is a hardy non-edible oil-seed plant that can sustain harsh environments, adapt well to semi-arid marginal, and wastelands and hence has been treated as a potential alternative energy. The plant growth promoting N-fixer can improve the growth of Jatropha on N-limiting soils (>40%). This prompted us to sequence the genome of these strains and, via comparative genomics, identify functions essential for the successful colonization, biological nitrogen fixation and endophytic association with its Jatropha host under N-limiting conditions. Analysis of the genome sequence, combined with metabolite analysis and quantitative PCR, pointed to a remarkable interaction between N-fixer and its Jatropha host with the endophyte responsible for the production of a phytohormone, and a precursor for another that Jatropha is unable to synthesize, and where the production of the plant growth promoting compounds depended on the presence of plant synthesized compounds, such as sucrose, in the growth medium. Our results provide the basis to better understanding the synergistic interactions between Jatropha and N-fixer. This information can be further exploited to improve establishment and sustainable production of Jatropha on N-limiting soils using endophytic bacteria such as Enterobacter and Methylobacterium as growth promoting agents.  
Is JGI Project
  
Project Information Visibility Public
PROJECT TYPE
Specimen Organism
Nucleic Acid DNA
Sequencing Strategy
Whole Genome Sequencing  
EXTERNAL PROJECT LINKS
Project Information
  
Database
  
Genome Publications Genome announcements Complete Genome Sequence of Enterobacter sp. Strain R4-368, an Endophytic N-Fixing Gammaproteobacterium Isolated from Surface-Sterilized Roots of Jatropha curcas L.

Other Publications
  
SRA Experiment
  
PROJECT COMPOSITION
Study Enterobacter sp. R4-368
Organism Enterobacter sp. R4-368
Analysis Projects Enterobacter sp. R4-368
Number of Analysis Projects 1
Number of DAPs
0  
SEQUENCING INFORMATION
Sequencing Status Complete
Sequencing Quality (MIGS-31) Level 6: Finished
Finishing Goal
  
Sequencing Comments
  
Sequencing Technology (MIGS-29) 454 GS FLX Titanium
Illumina
Library Method (MIGS-28)
3 kb mate-paired library  
Number Of Reads
130,133  
Read Size
  
Vector
  
GC Percent
54  
Chromosome Count
1  
Plasmid Count
1  
ORGANISM NAME
GOLD Organism ID Go0036960
Organism Name
Enterobacter sp. R4-368  
Other names (alias, synonyms, short names, common names)
  
Organism Domain
BACTERIAL  
Phylogeny
PROTEOBACTERIA-GAMMA  
Genus
Enterobacter  
Genus Synonyms
  
Species
Enterobacter sp. R4-368  
Subspecies
  
Species Synonyms
  
Strain
R4-368  
Strain Synonyms
  
Serovar/Cultivar
  
Culture Collection ID
  
Type Strain
Unknown  
Exemplar DOI
  
Exemplar Name
  
Taxon DOI
  
Biosafety Level
  
Organism Comments
  
Cultured
Yes  
Culture Type
Isolate  
Organism Type
Natural  
Uncultured Type
  
Commercial Strain
  
Commercial Strain Comments
  
ORGANISM TAXONOMY
NCBI Taxonomy ID 1166130
NCBI Superkingdom
Bacteria  
NCBI Kingdom
  
NCBI Phylum
Proteobacteria  
NCBI Class
Gammaproteobacteria  
NCBI Order
Enterobacterales  
NCBI Family
Enterobacteriaceae  
NCBI Genus
Enterobacter  
NCBI Species
Enterobacter sp. R4-368  
ISOLATION CLASSIFICATION
Ecosystem
Host-associated  
Ecosystem Category
Plants  
Ecosystem Type
Rhizoplane  
Ecosystem Subtype
Unclassified  
Specific Ecosystem
Unclassified  
ORGANISM EXTERNAL REFERENCES
Strain Info ID
Genbank 16S ID
GENERAL PROPERTIES
Oxygen Requirement
Aerobe  
Cell Shape
Rod-shaped  
Motility
Motile  
Sporulation
Nonsporulating  
Temperature Range
Mesophile  
Temperature Optimum
30C  
Salinity
  
pH
7  
Cell Diameter
0.55 um  
Cell Length
2.11 um  
Color
Cream  
Gram Staining
Gram+  
Biotic Relationships Symbiotic
Symbiotic Physical Interaction
Endosymbiotic extracellular  
Symbiotic Relationship
Commensal  
Symbiont Name
  
Symbiont Taxon ID
  
Cell Arrangements Singles
Pairs
Diseases None
Known Habitats Root nodule
Host
Metabolisms Nitrogen fixation
Phenotypes Nitrogen cycle
Energy Sources Heterotroph
CYANOBACTERIA METADATA
Filaments
  
Filament Type
  
Differentiated Cells
  
Position Of Heterocysts
  
Cysts
  
Mode Of Reproduction
  
Trichome
  
Trichome Type
  
Carotenoids
  
Pigments
  
Phage Infectivity
  
Phage Type
  
Restriction Enzyme
  
Toxin Production
  
Toxin Type
  
Secondary Metabolites
  
Developmental Cycle
  
Branching
  
Gas Vesicles
  
Gas Vesicle Type
  
Diagnostic Inclusion Body Information
  
Extracellular Structures
  
Bloom Forming
  
Pigment Type
  
Complementary chromatic adaptation (CCA)
  
Medium Types
  

 

 

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