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SRA STUDY
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SRA Study Id
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ERP023074
(Link to NCBI )
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Study Title
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Detection of bacterial pathogens from broncho-alveolar lavage by next-generation sequencing
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SRA Experiments
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| SRA Experiment Id |
ERX2049752
(Link to NCBI ) |
| Experiment Title |
Illumina HiSeq 2000 paired end sequencing |
| Exp Library Strategy |
WGS |
| Library Source |
METAGENOMIC |
| Library Selection |
RANDOM |
| Library Name |
unspecified |
| Library Layout |
PAIRED |
| Library Instrument |
ILLUMINA_HI_SEQ_2000 |
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| SRA Experiment Id |
ERX2049751
(Link to NCBI ) |
| Experiment Title |
Illumina HiSeq 2500 paired end sequencing |
| Exp Library Strategy |
WGS |
| Library Source |
METAGENOMIC |
| Library Selection |
RANDOM |
| Library Name |
unspecified |
| Library Layout |
PAIRED |
| Library Instrument |
ILLUMINA_HI_SEQ_2500 |
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Study Abstract
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The application of culture-free methods, notably whole-metagenome shotgun (WMGS) sequencing, in routine clinical analysis is still limited although they have some advantage over culture-based approach. A combination of an appropriate DNA extraction procedure, sequencing and bioinformatics tools is essential for removal of human DNA and improved bacterial species identification. We tackled these issues by applying a specifically-designed clinical metagenomics pipeline, from DNA extraction to bioinformatics methods, on a broncho-alveolar lavage (BAL) sample from a severely immunocompromised patient who developed an atypical nosocomial pneumonia.
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Alias
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ena-STUDY-GUH-16-05-2017-14:21:20:116-366
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External Id
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BioProject=PRJEB20877
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