PROJECT INFORMATION
GOLD Project ID
Gp0101051  
Project Name
Richelia intracellularis RC01  
Other Names
  
Legacy ER Project ID
76466  
Legacy GOLD ID
Gi0076466  
NCBI BioProject Name Richelia intracellularis RC01
NCBI BioProject ID 243336
NCBI BioProject Accession PRJEB5207
NCBI Locus Tag
RINTRC  
NCBI BioSample Accession SAMEA3138990
PI
Added By JGI automated process on 2014-07-25
Last Modified By Supratim Mukherjee on 2017-03-29
Project Comments
  
Project Status
Permanent Draft  
Project Relevance
  
Sequencing Center University of California, Santa Cruz (USA)
Collaborating Institute
  
Funding Agency
  
Project Description
Heterocyst-forming cyanobacteria form associations with a wide variety of eukaryotic hosts, including marine diatoms. The relationships of Nostoc spp. and Anabaena spp. with multicellular plants are well-studied, but little is known about diatom-diazotroph associations (DDAs). The symbiont of the marine diatom Hemiaulus hauckii was recently shown to have a streamlined genome, especially within nitrogen metabolism pathways. Richelia is also commonly found in oligotrophic open ocean environments associated with the diatom Rhizosolenia clevei. We obtained a draft genome of R. intracellularis from cells separated from the host diatom R. clevei by flow cytometry sorting. The genome of R. intracellularis has low percent coding (64%) relative to the sequenced of heterocyst-forming cyanobacteria (averages of 80% and 41%, respectively), but not as low as the H. hauckii symbiont (56%). Additionally, the R. clevei symbiont lacks several genes encoding for key nitrogen acquisition proteins, like the H. hauckii symbiont. However, a gene encoding glutamate synthase is present in the R. clevei symbiont genome. Hypotheses regarding the symbiont evolution, interactions between host and symbiont, and the implications for cycling of nitrogen and carbon in the open ocean are considered.  
Is JGI Project
No  
Project Information Visibility Public
PROJECT TYPE
Specimen Organism
Nucleic Acid DNA
Sequencing Strategy
Whole Genome Sequencing  
EXTERNAL PROJECT LINKS
Project Information
  
Database
  
Genome Publications
  
Other Publications
  
SRA Experiment
  
PROJECT COMPOSITION
Study The genome sequence of the heterocyst-forming cyanobacterial symbiont of the diatom Rhizosolenia
Organism Richelia intracellularis RC01
Analysis Projects Richelia intracellularis RC01
Number of Analysis Projects (AP)
1  
Number of Derived Analyis Projects (DAP)
0  
SEQUENCING INFORMATION
Sequencing Status Complete
Sequencing Quality (MIGS-31)
Finishing Goal
  
Sequencing Comments
  
Sequencing Technology (MIGS-29)
  
Library Method (MIGS-28)
  
Number Of Reads
  
Read Size
  
Vector
  
GC Percent
39  
Chromosome Count
  
Plasmid Count
  
ORGANISM NAME
GOLD Organism ID Go0101051
Organism Name
Richelia intracellularis RC01  
Other names (alias, synonyms, short names, common names)
  
Organism Domain
BACTERIAL  
Phylogeny
CYANOBACTERIA  
Genus
Richelia  
Genus Synonyms
  
Species
Richelia intracellularis  
Subspecies
  
Species Synonyms
  
Strain
RC01  
Strain Synonyms
  
Serovar/Cultivar
  
Culture Collection ID
  
Type Strain
Unknown  
Exemplar DOI
  
Exemplar Name
  
Taxon DOI
  
Biosafety Level
  
Organism Comments
  
Cultured
Yes  
Culture Type
Isolate  
Organism Type
Natural  
Uncultured Type
  
Commercial Strain
  
Commercial Strain Comments
  
ORGANISM TAXONOMY
NCBI Taxonomy ID 1164990
NCBI Superkingdom
Bacteria  
NCBI Kingdom
  
NCBI Phylum
Cyanobacteria  
NCBI Class
  
NCBI Order
Nostocales  
NCBI Family
Nostocaceae  
NCBI Genus
Richelia  
NCBI Species
Richelia intracellularis  
ISOLATION CLASSIFICATION
Ecosystem
  
Ecosystem Category
  
Ecosystem Type
  
Ecosystem Subtype
  
Specific Ecosystem
  
ORGANISM EXTERNAL REFERENCES
Strain Info ID
Genbank 16S ID
GENERAL PROPERTIES
Oxygen Requirement
  
Cell Shape
  
Motility
  
Sporulation
  
Temperature Range
  
Salinity
  
pH
  
Cell Diameter
  
Cell Length
  
Color
  
Gram Staining
  
Biotic Relationships
  
Symbiotic Physical Interaction
  
Symbiotic Relationship
  
Symbiont Name
  
Symbiont Taxon ID
  
Cell Arrangements
  
Diseases
  
Known Habitats
  
Metabolisms
  
Phenotypes
  
Energy Sources
  

 

 

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