PROJECT INFORMATION
GOLD Project ID
Gp0088990  
Project Name
Planomonospora alba NRRL 18924  
Other Names
Planomonospora alba planosporicin biosynthesis gene cluster, strain NRRL 18924  
Legacy ER Project ID
71364  
Legacy GOLD ID
Gi0071364  
NCBI BioProject Name
  
NCBI BioProject ID
NCBI BioProject Accession
NCBI Locus Tag
  
NCBI BioSample Accession
PI Emma Sherwood
Added By Michelle Isbandi on 2014-03-10
Last Modified By JGI automated process on 2014-05-20
Project Comments
The increasing prevalence of antibiotic resistance in bacterial pathogens has renewed focus on natural products with antimicrobial properties. Lantibiotics are ribosomally synthesized peptide antibiotics that are posttranslationally modified to introduce (methyl)lanthionine bridges. Actinomycetes are renowned for their ability to produce a large variety of antibiotics, many with clinical applications, but are known to make only a few lantibiotics. One such compound is planosporicin produced by Planomonospora alba, which inhibits cell wall biosynthesis in Gram-positive pathogens. Planosporicin is a type AI lantibiotic structurally similar to those which bind lipid II, the immediate precursor for cell wall biosynthesis. The gene cluster responsible for planosporicin biosynthesis was identified by genome mining and subsequently isolated from a P. alba cosmid library. A minimal cluster of 15 genes sufficient for planosporicin production was defined by heterologous expression in Nonomuraea sp. strain ATCC 39727, while deletion of the gene encoding the precursor peptide from P. alba, which abolished planosporicin production, was also used to confirm the identity of the gene cluster. Deletion of genes encoding likely biosynthetic enzymes identified through bioinformatic analysis revealed that they, too, are essential for planosporicin production in the native host. Reverse transcription-PCR (RT-PCR) analysis indicated that the planosporicin gene cluster is transcribed in three operons. Expression of one of these, pspEF, which encodes an ABC transporter, in Streptomyces coelicolor A3(2) conferred some degree of planosporicin resistance on the heterologous host. The inability to delete these genes from P. alba suggests that they play an essential role in immunity in the natural producer.  
Project Status
Permanent Draft  
Project Relevance Pharmaceutical
Medical
Sequencing Center
  
Collaborating Institute McMaster University (Canada)
Funding Agency
  
Project Description
  
Project Information Visibility Public
PROJECT TYPE
Specimen Organism
Nucleic Acid
  
Sequencing Strategy
Genome fragments  
EXTERNAL PROJECT LINKS
Project Information
  
Database
  
Genome Publications Journal of bacteriology 195(10):2309-21 Cloning and analysis of the planosporicin lantibiotic biosynthetic gene cluster of Planomonospora alba.
Other Publications
  
PROJECT COMPOSITION
Study Encyclopedia of Biosynthetic Clusters (EBiC)
Organism Planomonospora alba NRRL 18924
Analysis Projects Planomonospora alba NRRL 18924
Number of Analysis Projects 1
Number of DAPs
0  
SEQUENCING INFORMATION
Sequencing Status Complete
Sequencing Quality (MIGS-31)
Finishing Goal
  
Sequencing Comments
  
Sequencing Technology (MIGS-29) 454
Library Method (MIGS-28)
  
Number Of Reads
  
Read Size
  
Vector
  
GC Percent
74  
Chromosome Count
  
Plasmid Count
  
ORGANISM NAME
GOLD Organism ID Go0088990
Organism Name
Planomonospora alba NRRL 18924  
Other names (alias, synonyms, short names, common names)
  
Organism Domain
BACTERIAL  
Phylogeny
ACTINOBACTERIA  
Genus
Planomonospora  
Genus Synonyms
  
Species
Planomonospora alba  
Subspecies
  
Species Synonyms
  
Strain
NRRL 18924  
Strain Synonyms
  
Serovar-Biovar
  
Culture Collection ID
NRRL 18924  
Type Strain
Yes  
Exemplar DOI
10.1601/ex.7518  
Exemplar Name
Planomonospora alba  
Taxon DOI
10.1601/tx.7518  
Biosafety Level
1  
Organism Comments
  
Cultured
Yes  
Culture Type
Isolate  
Organism Type
Natural  
Uncultured Type
  
Commercial Strain
  
Commercial Strain Comments
  
ORGANISM TAXONOMY
NCBI Taxonomy ID 161354
NCBI Superkingdom
Bacteria  
NCBI Kingdom
  
NCBI Phylum
Actinobacteria  
NCBI Class
Actinobacteria  
NCBI Order
Streptosporangiales  
NCBI Family
Streptosporangiaceae  
NCBI Genus
Planomonospora  
NCBI Species
Planomonospora alba  
ISOLATION CLASSIFICATION
Ecosystem
  
Ecosystem Category
  
Ecosystem Type
  
Ecosystem Subtype
  
Specific Ecosystem
  
ORGANISM EXTERNAL REFERENCES
Strain Info ID
Genbank 16S ID
GENERAL PROPERTIES
Oxygen Requirement
  
Cell Shape
  
Motility
  
Sporulation
  
Temperature Range
  
Temperature Optimum
  
Salinity
  
pH
  
Cell Diameter
  
Cell Length
  
Color
  
Gram Staining
Gram+  
Biotic Relationships
  
Symbiotic Physical Interaction
  
Symbiotic Relationship
  
Symbiont Name
  
Symbiont Taxon ID
  
Cell Arrangements
  
Diseases
  
Known Habitats
  
Metabolisms
  
Phenotypes
  
Energy Sources
  
CYANOBACTERIA METADATA
Filaments
  
Filament Type
  
Differentiated Cells
  
Position Of Heterocysts
  
Cysts
  
Mode Of Reproduction
  
Trichome
  
Trichome Type
  
Carotenoids
  
Pigments
  
Phage Infectivity
  
Phage Type
  
Restriction Enzyme
  
Toxin Production
  
Toxin Type
  
Secondary Metabolites
  
Developmental Cycle
  
Branching
  
Gas Vesicles
  
Gas Vesicle Type
  
Diagnostic Inclusion Body Information
  
Extracellular Structures
  
Bloom Forming
  
Pigment Type
  
Complementary chromatic adaptation (CCA)
  
Medium Types
  

 

 

  You are going to add the following user to your project, biosample and study.

 

Contact Name/Email:

Do you want to continue?

 

 

  You are going to remove permissions for the following user to your project, biosample and study.

 

Contact Name/Email:

Do you want to continue?

 

 

  Changing the SP visibility will also change visibility on the following items:

 

Do you want to continue with this visibility change?

 

 

  Checking that this Sequencing Project can be made private:

 

Do you want to continue with this visibility change?