PROJECT INFORMATION
GOLD Project ID
Gp0037464  
Project Name
Chlamydia trachomatis IU824  
Other Names
  
Legacy ER Project ID
43391  
Legacy GOLD ID
Gc0043391  
NCBI BioProject Name Chlamydia trachomatis IU824
NCBI BioProject ID 179054
NCBI BioProject Accession PRJEB223
NCBI Locus Tag
  
NCBI BioSample Accession
PI
Added By JGI automated process on 2013-04-02
Last Modified By Jon Bertsch on 2014-04-08
Project Comments
Chlamydia trachomatis is the most common bacterial sexually transmitted infection worldwide and the leading cause of preventable blindness in developing countries. Tetracycline is commonly the drug of choice for treating C. trachomatis infections, but cases of antibiotic resistance in clinical isolates have previously been reported. Here, we used antibiotic resistance assays and whole-genome sequencing to interrogate the hypothesis that two clinical isolates (IU824 and IU888) have acquired mechanisms of antibiotic resistance. Immunofluorescence staining was used to identify C. trachomatis inclusions in cell cultures grown in the presence of tetracycline; however, only antibiotic-free control cultures yielded the strong fluorescence associated with the presence of chlamydial inclusions. Infectivity was lost upon passage of harvested cultures grown in the presence of tetracycline into antibiotic-free medium, so we conclude that these isolates were phenotypically sensitive to tetracycline. Comparisons of the genome and plasmid sequences for the two isolates with tetracycline-sensitive strains did not identify regions of low sequence identity that could accommodate horizontally acquired resistance genes, and the tetracycline binding region of the 16S rRNA gene was identical to that of the sensitive control strains. The porB gene of strain IU824, however, was found to contain a premature stop codon not previously identified, which is noteworthy but unlikely to be related to tetracycline resistance. In conclusion, we found no evidence of tetracycline resistance in the two strains investigated, and it seems most likely that the small, aberrant inclusions previously identified resulted from the high chlamydial load used in the original antibiotic resistance assays.  
Project Status
Complete and Published  
Project Relevance Human Pathogen
Sequencing Center University of Southampton
Collaborating Institute
  
Funding Agency
  
Project Description
  
Is JGI Project
  
Project Information Visibility Public
PROJECT TYPE
Specimen Organism
Nucleic Acid DNA
Sequencing Strategy
Whole Genome Sequencing  
EXTERNAL PROJECT LINKS
Project Information
  
Database
  
Genome Publications Microbiology (Reading, Engl.) 159(Pt 4):748-56 Chlamydia trachomatis clinical isolates identified as tetracycline resistant do not exhibit resistance in vitro: whole-genome sequencing reveals a mutation in porB but no evidence for tetracycline resistance genes.

Other Publications
  
SRA Experiment
  
PROJECT COMPOSITION
Study Genome and plasmid sequences of C. trachomatis strain IU824 previously identified as being resistant to tetracycline.
Organism Chlamydia trachomatis IU824
Analysis Projects Chlamydia trachomatis IU824
Number of Analysis Projects 1
Number of DAPs
0  
SEQUENCING INFORMATION
Sequencing Status Complete
Sequencing Quality (MIGS-31) Level 6: Finished
Finishing Goal
  
Sequencing Comments
  
Sequencing Technology (MIGS-29) Illumina HiSeq 2000
Library Method (MIGS-28)
  
Number Of Reads
  
Read Size
  
Vector
  
GC Percent
41  
Chromosome Count
1  
Plasmid Count
1  
ORGANISM NAME
GOLD Organism ID Go0037464
Organism Name
Chlamydia trachomatis IU824  
Other names (alias, synonyms, short names, common names)
  
Organism Domain
BACTERIAL  
Phylogeny
CHLAMYDIAE  
Genus
Chlamydia  
Genus Synonyms
  
Species
Chlamydia trachomatis  
Subspecies
  
Species Synonyms
  
Strain
IU824  
Strain Synonyms
  
Serovar/Cultivar
  
Culture Collection ID
  
Type Strain
Unknown  
Exemplar DOI
  
Exemplar Name
  
Taxon DOI
  
Biosafety Level
  
Organism Comments
  
Cultured
Yes  
Culture Type
Isolate  
Organism Type
Natural  
Uncultured Type
  
Commercial Strain
  
Commercial Strain Comments
  
ORGANISM TAXONOMY
NCBI Taxonomy ID 1260222
NCBI Superkingdom
Bacteria  
NCBI Kingdom
  
NCBI Phylum
Chlamydiae  
NCBI Class
Chlamydiia  
NCBI Order
Chlamydiales  
NCBI Family
Chlamydiaceae  
NCBI Genus
Chlamydia  
NCBI Species
Chlamydia trachomatis  
ISOLATION CLASSIFICATION
Ecosystem
  
Ecosystem Category
  
Ecosystem Type
  
Ecosystem Subtype
  
Specific Ecosystem
  
ORGANISM EXTERNAL REFERENCES
Strain Info ID
Genbank 16S ID
GENERAL PROPERTIES
Oxygen Requirement
  
Cell Shape
  
Motility
  
Sporulation
  
Temperature Range
  
Temperature Optimum
  
Salinity
  
pH
  
Cell Diameter
  
Cell Length
  
Color
  
Gram Staining
  
Biotic Relationships
  
Symbiotic Physical Interaction
  
Symbiotic Relationship
  
Symbiont Name
  
Symbiont Taxon ID
  
Cell Arrangements
  
Diseases
  
Known Habitats
  
Metabolisms
  
Phenotypes
  
Energy Sources
  
CYANOBACTERIA METADATA
Filaments
  
Filament Type
  
Differentiated Cells
  
Position Of Heterocysts
  
Cysts
  
Mode Of Reproduction
  
Trichome
  
Trichome Type
  
Carotenoids
  
Pigments
  
Phage Infectivity
  
Phage Type
  
Restriction Enzyme
  
Toxin Production
  
Toxin Type
  
Secondary Metabolites
  
Developmental Cycle
  
Branching
  
Gas Vesicles
  
Gas Vesicle Type
  
Diagnostic Inclusion Body Information
  
Extracellular Structures
  
Bloom Forming
  
Pigment Type
  
Complementary chromatic adaptation (CCA)
  
Medium Types
  

 

 

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