PROJECT INFORMATION
GOLD Project ID
Gp0036910  
Project Name
Methylobacterium sp. L2-4  
Other Names
  
Legacy ER Project ID
42860  
Legacy GOLD ID
Gi0042860  
NCBI BioProject Name Methylobacterium sp. L2-4
NCBI BioProject ID 194465
NCBI BioProject Accession PRJNA194465
NCBI Locus Tag
J443  
NCBI BioSample Accession SAMN02471039
PI Madhaiyan Munusamy
Added By Tatiparthi Reddy on 2013-03-29
Last Modified By JGI automated process on 2014-08-18
Project Comments
  
Project Status
Permanent Draft  
Project Relevance Nitrogen fixation
Agricultural
Sequencing Center Macrogen (USA)
Collaborating Institute
  
Funding Agency Temasek Trust (Singapore)
Project Description
Jatropha (Jatropha curcas L.) is a hardy non-edible oil-seed plant that can sustain harsh environments, adapt well to semi-arid marginal, and wastelands and hence has been treated as a potential alternative energy. The plant growth promoting N-fixer can improve the growth of Jatropha on N-limiting soils (>40%). This prompted us to sequence the genome of these strains and, via comparative genomics, identify functions essential for the successful colonization, biological nitrogen fixation and endophytic association with its Jatropha host under N-limiting conditions. Analysis of the genome sequence, combined with metabolite analysis and quantitative PCR, pointed to a remarkable interaction between N-fixer and its Jatropha host with the endophyte responsible for the production of a phytohormone, and a precursor for another that Jatropha is unable to synthesize, and where the production of the plant growth promoting compounds depended on the presence of plant synthesized compounds, such as sucrose, in the growth medium. Our results provide the basis to better understanding the synergistic interactions between Jatropha and N-fixer. This information can be further exploited to improve establishment and sustainable production of Jatropha on N-limiting soils using endophytic bacteria such as Enterobacter and Methylobacterium as growth promoting agents.  
Project Information Visibility Public
PROJECT TYPE
Specimen Organism
Nucleic Acid DNA
Sequencing Strategy
Whole Genome Sequencing  
EXTERNAL PROJECT LINKS
Project Information
  
Database
  
Genome Publications
  
Other Publications
  
PROJECT COMPOSITION
Study Methylobacterium sp. L2-4
Organism Methylobacterium sp. L2-4
Analysis Projects Methylobacterium sp. L2-4
Number of Analysis Projects 1
Number of DAPs
0  
SEQUENCING INFORMATION
Sequencing Status Complete
Sequencing Quality (MIGS-31) Level 4: Annotation-Directed
Finishing Goal
  
Sequencing Comments
  
Sequencing Technology (MIGS-29) 454 GS FLX Titanium
Illumina
Library Method (MIGS-28)
  
Number Of Reads
273,341  
Read Size
  
Vector
  
GC Percent
71  
Chromosome Count
  
Plasmid Count
  
ORGANISM NAME
GOLD Organism ID Go0036910
Organism Name
Methylobacterium sp. L2-4  
Other names (alias, synonyms, short names, common names)
  
Organism Domain
BACTERIAL  
Phylogeny
PROTEOBACTERIA-ALPHA  
Genus
Methylobacterium  
Genus Synonyms
  
Species
Methylobacterium sp. L2-4  
Subspecies
  
Species Synonyms
  
Strain
L2-4  
Strain Synonyms
  
Serovar-Biovar
  
Culture Collection ID
  
Type Strain
Unknown  
Exemplar DOI
  
Exemplar Name
  
Taxon DOI
  
Biosafety Level
  
Organism Comments
  
Cultured
Yes  
Culture Type
Isolate  
Organism Type
Natural  
Uncultured Type
  
Commercial Strain
  
Commercial Strain Comments
  
ORGANISM TAXONOMY
NCBI Taxonomy ID 1166158
NCBI Superkingdom
Bacteria  
NCBI Kingdom
  
NCBI Phylum
Proteobacteria  
NCBI Class
Alphaproteobacteria  
NCBI Order
Rhizobiales  
NCBI Family
Methylobacteriaceae  
NCBI Genus
Methylobacterium  
NCBI Species
Methylobacterium sp. L2-4  
ISOLATION CLASSIFICATION
Ecosystem
Host-associated  
Ecosystem Category
Plants  
Ecosystem Type
Phylloplane  
Ecosystem Subtype
Unclassified  
Specific Ecosystem
Unclassified  
ORGANISM EXTERNAL REFERENCES
Strain Info ID
Genbank 16S ID
GENERAL PROPERTIES
Oxygen Requirement
Aerobe  
Cell Shape
Rod-shaped  
Motility
  
Sporulation
Nonsporulating  
Temperature Range
Mesophile  
Temperature Optimum
30C  
Salinity
  
pH
7  
Cell Diameter
  
Cell Length
  
Color
Pink  
Gram Staining
Gram+  
Biotic Relationships Symbiotic
Symbiotic Physical Interaction
Endosymbiotic extracellular  
Symbiotic Relationship
Commensal  
Symbiont Name
  
Symbiont Taxon ID
  
Cell Arrangements Singles
Pairs
Diseases
  
Known Habitats Host
Metabolisms
  
Phenotypes
  
Energy Sources
  
CYANOBACTERIA METADATA
Filaments
  
Filament Type
  
Differentiated Cells
  
Position Of Heterocysts
  
Cysts
  
Mode Of Reproduction
  
Trichome
  
Trichome Type
  
Carotenoids
  
Pigments
  
Phage Infectivity
  
Phage Type
  
Restriction Enzyme
  
Toxin Production
  
Toxin Type
  
Secondary Metabolites
  
Developmental Cycle
  
Branching
  
Gas Vesicles
  
Gas Vesicle Type
  
Diagnostic Inclusion Body Information
  
Extracellular Structures
  
Bloom Forming
  
Pigment Type
  
Complementary chromatic adaptation (CCA)
  
Medium Types
  

 

 

  You are going to add the following user to your project, biosample and study.

 

Contact Name/Email:

Do you want to continue?

 

 

  You are going to remove permissions for the following user to your project, biosample and study.

 

Contact Name/Email:

Do you want to continue?

 

 

  Changing the SP visibility will also change visibility on the following items:

 

Do you want to continue with this visibility change?

 

 

  Checking that this Sequencing Project can be made private:

 

Do you want to continue with this visibility change?