PROJECT INFORMATION
GOLD Project ID
Gp0322500  
Project Name
Natronobacterium gregoryi SP2  
Other Names
  
Legacy GOLD ID
  
NCBI BioProject Name Natronobacterium gregoryi SP2
NCBI BioProject ID 423232
NCBI BioProject Accession PRJNA423232
NCBI Locus Tag
  
NCBI BioSample Accession SAMN08213458
PI Alejandro Rodriguez Mojica
Added By Alejandro Rodriguez Mojica on 2018-07-02
Last Modified By Supratim Mukherjee on 2018-07-06
Project Comments
  
Project Status
Permanent Draft  
Project Relevance
  
Sequencing Center University of Electronic Science and Technology of China (UESTC) (China)
Collaborating Institute
  
Funding Agency
  
Project Description
The Natronobacterium gregoryi Argonaute (NgAgo)-guide DNA (gDNA) system was reported to have powerful genome editing capacity by Han Chunyu group. However, many related studies have shown no genome editing. For example, recently Liang X. group firstly demonstrated the potential of NgAgo/gDNA in inhibiting HBV replication through accelerating pgRNA degradation, but not DNA editing. Therefore, we grew this archaea and then sequenced the whole genome. The existing submissions are not updated for one year already. We tried to analyse the endogenous oligonucleotides-guide DNA and locate it in the genome. Here we sequenced the whole genome of Natronobacterium gregoryi SP2  
Is JGI Project
  
Project Information Visibility Public
PROJECT TYPE
Specimen Organism
Nucleic Acid DNA
Sequencing Strategy
Whole Genome Sequencing  
EXTERNAL PROJECT LINKS
Project Information
  
Database
  
Genome Publications
  
Other Publications
  
SRA Experiment
 
SRA Experiment Id SRX3511061
Experiment Title WGS of Natronobacterium gregoryi SP2
Exp Library Strategy WGS
Library Source GENOMIC
Library Selection PCR
Library Name N.gregoryiSP2
Library Layout PAIRED
Library Instrument ILLUMINA_HI_SEQ_4000
PROJECT COMPOSITION
Study Natronobacterium gregoryi SP2 Genome sequencing
Organism Natronobacterium gregoryi SP2
Analysis Projects Natronobacterium gregoryi SP2
Number of Analysis Projects 1
Number of DAPs
0  
SEQUENCING INFORMATION
Sequencing Status Complete
Sequencing Quality (MIGS-31) Level 1: Standard Draft
Finishing Goal Level 1: Standard Draft
Sequencing Comments
  
Sequencing Technology (MIGS-29) Illumina HiSeq
Library Method (MIGS-28)
  
Number Of Reads
  
Read Size
  
Vector
  
GC Percent
  
Chromosome Count
  
Plasmid Count
  
ORGANISM NAME
GOLD Organism ID Go0006794
Organism Name
Natronobacterium gregoryi SP2  
Other names (alias, synonyms, short names, common names)
  
Organism Domain
ARCHAEAL  
Phylogeny
EURYARCHAEOTA-HALOBACTERIA  
Genus
Natronobacterium  
Genus Synonyms
  
Species
Natronobacterium gregoryi  
Subspecies
  
Species Synonyms
  
Strain
SP2  
Strain Synonyms
  
Serovar/Cultivar
  
Culture Collection ID
ATCC 43098  
Type Strain
Yes  
Exemplar DOI
10.1601/ex.345  
Exemplar Name
Natronobacterium gregoryi  
Taxon DOI
10.1601/tx.345  
Biosafety Level
1  
Organism Comments
  
Cultured
Yes  
Culture Type
Isolate  
Organism Type
Natural  
Uncultured Type
  
Commercial Strain
  
Commercial Strain Comments
  
ORGANISM TAXONOMY
NCBI Taxonomy ID 797304
NCBI Superkingdom
Archaea  
NCBI Kingdom
  
NCBI Phylum
Euryarchaeota  
NCBI Class
Halobacteria  
NCBI Order
Natrialbales  
NCBI Family
Natrialbaceae  
NCBI Genus
Natronobacterium  
NCBI Species
Natronobacterium gregoryi  
ISOLATION CLASSIFICATION
Ecosystem
Environmental  
Ecosystem Category
Aquatic  
Ecosystem Type
Freshwater  
Ecosystem Subtype
Lentic  
Specific Ecosystem
Unclassified  
ORGANISM EXTERNAL REFERENCES
Strain Info ID
Genbank 16S ID D87970
GENERAL PROPERTIES
Oxygen Requirement
Aerobe  
Cell Shape
  
Motility
  
Sporulation
  
Temperature Range
Mesophile  
Temperature Optimum
  
Salinity
Halophile  
pH
  
Cell Diameter
  
Cell Length
  
Color
  
Gram Staining
Gram-  
Biotic Relationships Free living
Symbiotic Physical Interaction
  
Symbiotic Relationship
  
Symbiont Name
  
Symbiont Taxon ID
  
Cell Arrangements
  
Diseases
  
Known Habitats Aquatic
Lake
Fresh water
Metabolisms
  
Phenotypes
  
Energy Sources
  

 

 

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