PROJECT INFORMATION
GOLD Project ID
Gp0023434  
Project Name
Dolichospermum circinale ACBU02  
Other Names
  
Legacy ER Project ID
27897  
Legacy GOLD ID
Gi19911  
NCBI BioProject Name Dolichospermum circinale ACBU02
NCBI BioProject ID 69805
NCBI BioProject Accession PRJNA69805
NCBI Locus Tag
I31  
NCBI BioSample Accession
PI
Added By JGI automated process on 2012-07-30
Last Modified By
Project Comments
  
Project Status
incomplete  
Project Relevance
  
Sequencing Center American University in Cairo (USA)
Collaborating Institute
  
Funding Agency
  
Project Description
Paralytic shellfish poisoning (PSP) is a potentially fatal syndrome associated with the consumption of shellfish that have accumulated saxitoxin (STX). STX is produced by microscopic marine dinoflagellate algae. Little is known about the origin and spread of saxitoxin genes in these under-studied eukaryotes. Fortuitously, some freshwater cyanobacteria also produce STX, providing an ideal model for studying its biosynthesis. Here we focus on saxitoxin-producing cyanobacteria and their non-toxic sisters to elucidate the origin of genes involved in the putative STX biosynthetic pathway. We generated a draft genome assembly of the saxitoxin-producing (STX+) cyanobacterium Anabaena circinalis ACBU02 and searched for 26 candidate saxitoxin¬≠genes (named sxtA to sxtZ) that were recently identified in the toxic strain Cylindrospermopsis raciborskii T3. We also generated a draft assembly of the non-toxic (STX?) sister Anabaena circinalis ACFR02 to aid the identification of saxitoxin-specific genes. Comparative phylogenomic analyses revealed that nine putative STX genes were horizontally transferred from non-cyanobacterial sources, whereas one key gene (sxtA) originated in STX+ cyanobacteria via two independent horizontal transfers followed by fusion. In total, of the 26 candidate saxitoxin-genes, 13 are of cyanobacterial provenance and are monophyletic among the STX+ taxa, four are shared amongst STX+ and STX-cyanobacteria, and the remaining nine genes are specific to STX+ cyanobacteria. Our results provide evidence that the assembly of STX genes in ACBU02 involved multiple HGT events from different sources followed presumably by coordination of the expression of foreign and native genes in the common ancestor of STX+ cyanobacteria. The ability to produce saxitoxin was subsequently lost multiple independent times resulting in a nested relationship of STX+ and STX? strains among Anabaena circinalis strains.  
Is JGI Project
  
Project Information Visibility Public
PROJECT TYPE
Specimen Organism
Nucleic Acid DNA
Sequencing Strategy
Whole Genome Sequencing  
EXTERNAL PROJECT LINKS
Project Information
  
Database
  
Genome Publications
  
Other Publications
  
SRA Experiment
  
PROJECT COMPOSITION
Study Dolichospermum circinale ACBU02
Organism Dolichospermum circinale ACBU02
Analysis Projects
  
Number of Analysis Projects 0
Number of DAPs
0  
SEQUENCING INFORMATION
Sequencing Status In progress
Sequencing Quality (MIGS-31)
Finishing Goal
  
Sequencing Comments
  
Sequencing Technology (MIGS-29)
  
Library Method (MIGS-28)
  
Number Of Reads
  
Read Size
  
Vector
  
GC Percent
  
Chromosome Count
  
Plasmid Count
  
ORGANISM NAME
GOLD Organism ID Go0009642
Organism Name
Dolichospermum circinale ACBU02  
Other names (alias, synonyms, short names, common names)
  
Organism Domain
BACTERIAL  
Phylogeny
CYANOBACTERIA  
Genus
Dolichospermum  
Genus Synonyms
  
Species
Dolichospermum circinale  
Subspecies
  
Species Synonyms
  
Strain
ACBU02  
Strain Synonyms
  
Serovar/Cultivar
  
Culture Collection ID
  
Type Strain
No  
Exemplar DOI
  
Exemplar Name
  
Taxon DOI
  
Biosafety Level
  
Organism Comments
  
Cultured
Yes  
Culture Type
Isolate  
Organism Type
Natural  
Uncultured Type
  
Commercial Strain
  
Commercial Strain Comments
  
ORGANISM TAXONOMY
NCBI Taxonomy ID 1054832
NCBI Superkingdom
Bacteria  
NCBI Kingdom
  
NCBI Phylum
Cyanobacteria  
NCBI Class
  
NCBI Order
Nostocales  
NCBI Family
Aphanizomenonaceae  
NCBI Genus
Dolichospermum  
NCBI Species
Dolichospermum circinale  
ISOLATION CLASSIFICATION
Ecosystem
  
Ecosystem Category
  
Ecosystem Type
  
Ecosystem Subtype
  
Specific Ecosystem
  
ORGANISM EXTERNAL REFERENCES
Strain Info ID
Genbank 16S ID
GENERAL PROPERTIES
Oxygen Requirement
  
Cell Shape
  
Motility
  
Sporulation
  
Temperature Range
  
Temperature Optimum
  
Salinity
  
pH
  
Cell Diameter
  
Cell Length
  
Color
  
Gram Staining
Gram-  
Biotic Relationships
  
Symbiotic Physical Interaction
  
Symbiotic Relationship
  
Symbiont Name
  
Symbiont Taxon ID
  
Cell Arrangements
  
Diseases
  
Known Habitats
  
Metabolisms
  
Phenotypes
  
Energy Sources
  

 

 

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