PROJECT INFORMATION
GOLD Project ID
Gp0101027  
Project Name
Pseudomonas aeruginosa PA38182  
Other Names
  
Legacy ER Project ID
76442  
Legacy GOLD ID
Gi0076442, Gi0076442  
NCBI BioProject Name Genome sequencing and characterization of an XDR ST111 serotype O12 hospital outbreak strain of Pseudomonas aeruginosa
NCBI BioProject ID 236003
NCBI BioProject Accession PRJEB4573
NCBI Locus Tag
BN889  
NCBI BioSample Accession
PI
Added By JGI automated process on 2014-07-25
Last Modified By Michelle Isbandi on 2014-08-01
Project Comments
  
Project Status
Permanent Draft  
Project Relevance Pathogen
Medical
Sequencing Center St George's University of London
Collaborating Institute
  
Funding Agency
  
Project Description
A series of extensively drug-resistant (XDR) isolates of Pseudomonas aeruginosa from two outbreaks in UK hospitals were characterized by whole genome sequencing (WGS). Although these isolates were resistant to antibiotics other than colistin, we confirmed that they are still sensitive to disinfectants. The sequencing confirmed that isolates in the larger outbreak were serotype O12 and further revealed that they belonged to the ST111 sequence type which is a major epidemic strain of P. aeruginosa throughout Europe. As this is the first reported sequence of an ST111 strain, the genome was examined in depth, focusing particularly on antibiotic resistance and potential virulence genes, and on the reported regions of genome plasticity. High degrees of sequence similarity were discovered between outbreak isolates collected from recently infected patients, sinks, an isolate from the sewer and an historical isolate, suggesting that the ST111 strain has been endemic in the hospital for many years. The ability to translate easily from outbreak investigation to detailed genome biology using the same data demonstrates the flexibility of WGS application in a clinical setting.  
Project Information Visibility Public
PROJECT TYPE
Specimen Organism
Nucleic Acid DNA
Sequencing Strategy
Whole Genome Sequencing  
EXTERNAL PROJECT LINKS
Project Information
  
Database
  
Genome Publications
  
Other Publications
  
PROJECT COMPOSITION
Study Genome sequencing and characterization of an XDR ST111 serotype O12 hospital outbreak strain of Pseudomonas aeruginosa
Organism Pseudomonas aeruginosa PA38182
Analysis Projects Pseudomonas aeruginosa PA38182
Number of Analysis Projects 1
Number of DAPs
0  
SEQUENCING INFORMATION
Sequencing Status Complete
Sequencing Quality (MIGS-31) Level 2: High-Quality Draft
Finishing Goal
  
Sequencing Comments
  
Sequencing Technology (MIGS-29)
  
Library Method (MIGS-28)
  
Number Of Reads
  
Read Size
  
Vector
  
GC Percent
66  
Chromosome Count
1  
Plasmid Count
0  
ORGANISM NAME
GOLD Organism ID Go0101027
Organism Name
Pseudomonas aeruginosa PA38182  
Other names (alias, synonyms, short names, common names)
  
Organism Domain
BACTERIAL  
Phylogeny
PROTEOBACTERIA-GAMMA  
Genus
Pseudomonas  
Genus Synonyms
  
Species
Pseudomonas aeruginosa  
Subspecies
  
Species Synonyms
  
Strain
PA38182  
Strain Synonyms
  
Serovar-Biovar
  
Culture Collection ID
  
Type Strain
Unknown  
Exemplar DOI
  
Exemplar Name
  
Taxon DOI
  
Biosafety Level
  
Organism Comments
  
Cultured
Yes  
Culture Type
Isolate  
Organism Type
Natural  
Uncultured Type
  
Commercial Strain
  
Commercial Strain Comments
  
ORGANISM TAXONOMY
NCBI Taxonomy ID 1407059
NCBI Superkingdom
Bacteria  
NCBI Kingdom
  
NCBI Phylum
Proteobacteria  
NCBI Class
Gammaproteobacteria  
NCBI Order
Pseudomonadales  
NCBI Family
Pseudomonadaceae  
NCBI Genus
Pseudomonas  
NCBI Species
Pseudomonas aeruginosa  
ISOLATION CLASSIFICATION
Ecosystem
  
Ecosystem Category
  
Ecosystem Type
  
Ecosystem Subtype
  
Specific Ecosystem
  
ORGANISM EXTERNAL REFERENCES
Strain Info ID
Genbank 16S ID
GENERAL PROPERTIES
Oxygen Requirement
Aerobe  
Cell Shape
Coccus-shaped  
Motility
Motile  
Sporulation
  
Temperature Range
  
Temperature Optimum
  
Salinity
  
pH
  
Cell Diameter
  
Cell Length
  
Color
  
Gram Staining
Gram-  
Biotic Relationships
  
Symbiotic Physical Interaction
  
Symbiotic Relationship
  
Symbiont Name
  
Symbiont Taxon ID
  
Cell Arrangements
  
Diseases Opportunistic infection
Known Habitats Hospital
Human
Metabolisms
  
Phenotypes Catalase positive
Energy Sources
  
CYANOBACTERIA METADATA
Filaments
  
Filament Type
  
Differentiated Cells
  
Position Of Heterocysts
  
Cysts
  
Mode Of Reproduction
  
Trichome
  
Trichome Type
  
Carotenoids
  
Pigments
  
Phage Infectivity
  
Phage Type
  
Restriction Enzyme
  
Toxin Production
  
Toxin Type
  
Secondary Metabolites
  
Developmental Cycle
  
Branching
  
Gas Vesicles
  
Gas Vesicle Type
  
Diagnostic Inclusion Body Information
  
Extracellular Structures
  
Bloom Forming
  
Pigment Type
  
Complementary chromatic adaptation (CCA)
  
Medium Types
  

 

 

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